
AT is the main physiologic inhibitor of thrombin (activated factor XII, denoted conventionally as factor XIIa).1
AT forms an irreversible covalent bond between its arginine reactive site and the active serine site of thrombin. The result is an inactive 1:1 stoichiometric complex between the 2 molecules.2-4
When heparin binds with AT, a conformational change occurs to the AT molecule, exposing the reactive site and accelerating AT-thrombin complex formation approximately 1000-fold.2,3
Antithrombin Facts
| Synonyms | Antithrombin III heparin cofactor2,5,6 |
| Plasma concentration | 12.5 mg/dL4,7 |
| Functional type | Serpin2 |
| Inhibits | Thrombin, factors Xa, IXa, XIa, XIIa, kallikrein, and plasmin2 |
| Significance | One of the most important inhibitors of coagulation8 |
References:
- Escobar CE, Harmening DM, Joiner Maier DM, Simmons VL, Smith-Moore KM, Wyrick-Glatzel J. Introduction to hemostasis. In: Harmening DM, ed. Clinical Hematology and Fundamentals of Hemostasis. 4th ed. Philadelphia, PA: FA Davis Company; 2002:441-470.
- Kottke-Marchant K, Duncan A. Antithrombin deficiency: issues in laboratory diagnosis. Arch Pathol Lab Med. 2002;126(11):1326-1336.
- Rosenberg RD, Damus PS. The purification and mechanism of action of human antithrombin-heparin cofactor. J Biol Chem. 1973;248(18):6490-6505.
- Thrombate lll [prescribing information]. Research Triangle Park, NC:Talecris Biotherapeutics, Inc.;2009.
- Franchini M, Veneri D, Salvagno GL, Manzato F, Lippi G. Inherited thrombophilia. Crit Rev Clin Lab Sci. 2006;43(3):249-290.
- Collen D, Schetz J, de Cock F, Holmer E, Verstraete M. Metabolism of antithrombin III (heparin cofactor) in man: effects of venous thrombosis and of heparin administration. Eur J Clin Invest. 1977;7(1):27-35.
- Murano G, Williams L, Miller-Andersson M, Aronson DL, King C. Some properties of antithrombin-III and its concentration in human plasma. Thromb Res. 1980;18(1-2):259-262.
- Perry DJ. Antithrombin and its inherited deficiencies. Blood Rev. 1994;8(1):37-55.
